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Visual receptor cycle in normal and period mutant Drosophila: Microspectrophotometry, electrophysiology, and ultrastructural morphometry

Published online by Cambridge University Press:  02 June 2009

De-Mao Chen ,
J. Scott Christianson ,
Randall J. Sapp  and
William S. Stark
De-Mao Chen
Affiliation:
Division of Biological Sciences, The University of Missouri-Columbia, Columbia
J. Scott Christianson
Affiliation:
Division of Biological Sciences, The University of Missouri-Columbia, Columbia
Randall J. Sapp
Affiliation:
Division of Biological Sciences, The University of Missouri-Columbia, Columbia
William S. Stark
Affiliation:
Division of Biological Sciences, The University of Missouri-Columbia, Columbia
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Abstract

Visual pigment, sensitivity, and rhabdomere size were measured throughout a 12-h light/12-h dark cycle in Drosophila. Visual pigment and sensitivity were measured during subsequent constant darkness [dark/dark (D/D)]. MSP (microspectrophotometry) and the ERG (electroretinogram) revealed a cycling of visual pigment and sensitivity, respectively. A visual pigment decrease of 40% was noted at 4 h after light onset that recovered 2–4 h later in white-eyed (otherwise wild-type, w per+) flies. The ERG sensitivity [in w per+ flies in light/dark (L/D)] decreased by 75% at 4 h after light onset, more than expected if mediated by visual pigment (MSP) changes alone. ERG sensitivity begins decreasing 8 h before light onset while decreases in visual pigment begin 2 h after light onset. These cycles continue in constant darkness (D/D), suggesting a circadian rhythm. White-eyed period (per) mutants show similar cycles of visual pigment level and sensitivity in L/D; per's alterations, if any on the D/D cycles were subtle. The cross-sectional areas of rhabdomeres in w per+ were measured using electron micrographic (EM) morphometry. Area changed little through the L/D cycle.

Keywords

RhodopsinElectroretinogramSensitivityMicrospectrophotometryMorphometryTurnover

Information

Type
Research Articles
Information
Visual Neuroscience , Volume 9 , Issue 2 , August 1992 , pp. 125 - 135
Copyright
Copyright © Cambridge University Press 1992

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