Hostname: page-component-7dd5485656-c8zdz Total loading time: 0 Render date: 2025-10-31T17:34:51.633Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of advancement on nucleic acids in sugarbeet (Beta vulgaris) seeds

Published online by Cambridge University Press:  19 September 2008

M. Redfearn  and
D. J. Osborne
M. Redfearn*
Affiliation:
IACR-Broom's Barn, Higham, Bury St. Edmunds, Suffolk, IP28 6NP, UK
D. J. Osborne
Affiliation:
Oxford Research Unit, Open University, Foxcombe Hall, Oxford, OX1 5HR, UK
*
*Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Sugarbeet (Beta vulgaris) seeds which had undergone an advancement treatment germinated more quickly and showed greater germination in a standard test at 9°C than seeds which had been steeped in water. Both treatments included the fungicide, thiram (tetramethyl thiuram disulphide). Advanced seeds also emerged more quickly and showed higher establishment in the field. The levels of extractable high molecular weight (HMW) RNA and DNA from advanced, thiram-steeped and untreated seeds were compared. Advanced seeds contained significantly (P > 0.05) more HMW RNA and DNA than thiram-steeped or untreated seeds. Extractable DNA levels were higher from thiram-steeped seeds than untreated seeds but not significantly so. Cell-cycle studies on advanced seeds demonstrated an increase from 2% to 24% of root-tip nuclei with DNA contents of 4C and above. This indicates that DNA replication occurred during the advancement treatment. There was no evidence of cell division occurring, indicating that: (1) advanced seeds were arrested post-S-phase and (2) the period following DNA synthesis in sugarbeet seeds is desiccation tolerant.

Keywords

Beta vulgariscell cycleDNA synthesisprimed advancement seed treatmentRNA synthesis

Information

Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 7 , Issue 3 , September 1997 , pp. 261 - 268
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Ashraf, M. and Bray, C.M. (1993) DNA synthesis in osmoprimed leek (Allium porrum L.) seeds and evidence for repair and replication. Seed Science Research 3, 15–23.CrossRefGoogle Scholar
Baker, E.H. and Bradford, K.J. (1995) DNA content of tomato seeds during priming: relationship to germination rates and loss of storage life. pp 69–76in Fourth National Symposium on stand establishment of horticultural crops,Monterey, California.Google Scholar
Battle, J.P. and Whittington, W.J. (1969) The relation between inhibitory substances and variability in time to germination of sugar-beet clusters. Journal of Agricultural Science (Cambridge) 73, 337–346.CrossRefGoogle Scholar
Bernie, A.M.M. and Drennan, D.S.H. (1971) The effect of hydration-dehydration on seed germination. New Phytologist 70, 135–142.CrossRefGoogle Scholar
Bino, R.J., de Vries, J.N., Kraak, H.L. and van Pijlen, J.G. (1992) Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination. Annals of Botany 69, 231–236.CrossRefGoogle Scholar
Bray, C.M., Davison, P.A., Ashraf, M. and Taylor, R.M. (1989) Biochemical changes during osmopriming of leek seeds. Annals of Botany 63, 185–193.CrossRefGoogle Scholar
Bray, C.M., Ashraf, M., Davison, P.A. and Taylor, R.M. (1992) Molecular markers of seed quality. pp 887–896in Côme, D. and Corbineau, F. (Eds) Fourth International Workshop on seeds: basic and applied aspects of seed biologyVol. 3, Paris, ASFIS.Google Scholar
Clarke, N.A. and James, P.E. (1991) The effects of priming and accelerated ageing upon the nucleic acid content of leek seeds and their embryos. Journal of Experimental Botany 42, 261–268.CrossRefGoogle Scholar
Coolbear, P. and Grierson, D. (1979) Studies on the changes in the major nucleic acid components of tomato seeds (Lycopersicon esculentum Mill.) resulting from osmotic presowing treatment. Journal of Experimental Botany 30, 1153–1162.CrossRefGoogle Scholar
Coolbear, P., Grierson, D. and Heydecker, W. (1980) Osmotic pre-sowing treatments and nucleic acid accumulation in tomato seeds (Lycopersicon lycopersicum). Seed Science and Technology 8, 289–303.Google Scholar
Coolbear, P., Slater, R.J. and Bryant, J.A. (1990) Changes in nucleic acid levels associated with improved germination performance of tomato seeds after low temperature presowing treatment. Annals of Botany 65, 187–195.CrossRefGoogle Scholar
Crèvecoeur, M., Deltour, R. and Van de Walle, C. (1988) DNA content and nucleic acid synthesis in dehydrated maize embryos. Plant Physiology and Biochemistry 26, 65–71.Google Scholar
Cruz-García, F., González-Hernández, V.A., Molina-Moreno, J. and Vázquez-Ramos, J.M. (1995) Seed deterioration and respiration as related to DNA metabolism in germinating maize. Seed Science and Technology 23, 477–486.Google Scholar
Davison, P.A., Taylor, R.M. and Bray, C.M. (1991) Changes in ribosomal RNA integrity in leek (Allium porrum L.) seeds during osmopriming and drying-back treatments. Seed Science Research 1, 37–44.CrossRefGoogle Scholar
Deitch, A.D. (1966) Cytophotometry of nucleic acids. pp 327–349in Weid, D.L. (Ed.) Introduction to quantitative cytochemistry Vol. 1, New York, London, Academic Press.Google Scholar
Dell'Aquila, A. and Taranto, G. (1986) Cell division and DNA synthesis during osmopriming treatment and following germination in aged wheat embryos. Seed Science and Technology 14, 333–341.Google Scholar
Deltour, R. and Jacqmard, A. (1974) Relation between water stress and DNA synthesis during germination of Zea mays L. Annals of Botany 38, 529–534.CrossRefGoogle Scholar
De Tomasi, J.A. (1936) Improving the technic of the Feulgen stain. Stain Technology 11, 137–144.CrossRefGoogle Scholar
Durrant, M.J. and Jaggard, K.W. (1988) Sugar-beet seed advancement to increase establishment and decrease bolting. Journal of Agricultural Science (Cambridge) 110, 367–374.CrossRefGoogle Scholar
Durrant, M.J. and Mash, S.J. (1990) Sugar-beet seed treatments and early sowing. Seed Science and Technology 18, 839–850.Google Scholar
Durrant, M.J. and Mash, S.J. (1992) Sugar-beet treatments, water supply and depth of sowing. Annals of Applied Biology 120, 151–159.CrossRefGoogle Scholar
Durrant, M.J., Mash, S.J. and Jaggard, K.W. (1993) Effects of seed advancement and sowing date on establishment, bolting and yield of sugar beet. Journal of Agricultural Science (Cambridge) 121, 333–341.CrossRefGoogle Scholar
Ellis, R.H. and Roberts, E.H. (1981) The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9, 373–409.Google Scholar
Fu, J.R., Lu, X.H., Chen, R.Z., Zhang, B.Z., Liu, Z.S., Li, Z.S. and Cai, D.Y. (1988) Osmoconditioning of peanut (Arachis hypogea L.) seeds with PEG to improve vigour and some biochemical activities. Seed Science and Technology 16, 197–212.Google Scholar
Heydecker, W. and Coolbear, P. (1977) Seed treatments for improved performance — survey and attempted prognosis. Seed Science and Technology 5, 353–425.Google Scholar
ISTA (1979) Handbook for seedling evaluation Bekendam, J. and Grob, R. (Eds), Zürich, International Seed Testing Association.Google Scholar
ISTA (1990) Annexe to chapter 5: The germination test. Seed Science and Technology 18 supplement 1, 117.Google Scholar
Khan, A.A., Tao, K., Knypl, J.S., Borkowska, B. and Powell, L.E. (1978) Osmotic conditioning of seeds: physiological and biochemical changes. Acta Horticulturae 83, 267–278.CrossRefGoogle Scholar
Lanteri, S., Kraak, H.L., Ric de Vos, C.H. and Bino, R.J. (1993) Effects of osmotic preconditioning on nuclear replication activity in seeds of pepper (Capsicum annuum). Physiologia Plantarum 89, 433–440.CrossRefGoogle Scholar
Lanteri, S., Saracco, F., Kraak, H.L. and Bino, R.J. (1994) The effects of priming on nuclear replication activity and germination of pepper (Capsicum annuum) and tomato (Lycopersicon esculentum) seeds. Seed Science Research 4, 81–87.CrossRefGoogle Scholar
Saracco, F., Bino, R.J., Bergervoet, J.H.W. and Lanteri, S. (1995) Influence of priming-induced nuclear replication activity on storability of pepper (Capsicum annuum L.) seed. Seed Science Research 5, 25–29.CrossRefGoogle Scholar
Sen, S. and Osborne, D.J. (1974) Germination of rye embryos following hydration-dehydration treatments: enhancement of protein and RNA synthesis and earlier induction of DNA replication. Journal of Experimental Botany 25, 1010–1019.CrossRefGoogle Scholar
Thomas, T.H., Jaggard, K.W., Durrant, M.J., Mash, S.J. and Armstrong, M.J. (1993) Development of the sugar-beet seed advancement treatment in England. pp 437–448in Proceedings of the 56th Winter Congress of the Institut International Recherches de Betteravières,Brussels.Google Scholar
Thomas, T.H., Jaggard, K.W., Durrant, M.J. and Mash, S.J. (1994) The physiological advancement of sugarbeet seeds. pp 391—396in Martin, T.J. (Ed.) Seed treatment: progress and prospects. Monograph no. 57, Surrey, British Crop Protection Council.Google Scholar
Vollenweider, I. and Groscurth, P. (1992) Comparison of four DNA staining fluorescence dyes for measuring cell proliferation of lymphokine-activated killer (LAK) cells. Journal of Immunological Methods 149, 133–135.CrossRefGoogle ScholarPubMed