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Effects of osmotic priming on dormancy and storability of tomato (Lycopersicon esculentum Mill.) seeds

Published online by Cambridge University Press:  19 September 2008

Yongqing Liu ,
R. J. Bino ,
W. J. van der Burg ,
S. P. C. Groot  and
H. W. M. Hilhorst
Yongqing Liu
Affiliation:
Department of Horticulture, Hunan Agricultural University, Changsha 410128, China
R. J. Bino*
Affiliation:
Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
W. J. van der Burg
Affiliation:
Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
S. P. C. Groot
Affiliation:
Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
H. W. M. Hilhorst
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
*
*Correspondence
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Abstract

Freshly harvested tomato (Lycopersicon esculentum Mill, cv. Moneymaker) seeds were osmotically primed for 8 d in −1.0 MPa PEG-6000 solution and dried to about 6% water content for storage. Such so–called ‘fresh PEG priming’ enhanced seed germination and improved seedling performance as compared with the untreated control. Fresh PEG priming neither alleviated seed dormancy nor promoted DNA replication as was the case when seeds were dried upon harvest and subsequently primed in PEG (normal PEG priming). However, the addition of 10 μM GA4+7 to the osmotic priming solution triggered replicative DNA synthesis of fresh-priming seeds and further enhanced the germination process. After 5 months of storage in ambient temperature conditions, fresh PEG-primed seeds maintained more positive effects gained from priming, whereas, normal PEG-primed seeds had lost the promoting effects on germination. Normal PEG-primed seeds were much more susceptible to controlled deterioration than fresh PEG-primed seeds. It is suggested that the advancement of germination is negatively correlated with seed storability. The mechanisms of seed priming in relation to nuclear replication activities and physical changes are discussed.

Keywords

DNA replicationdormancyfree spacefresh primingtomato seed

Information

Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 6 , Issue 2 , June 1996 , pp. 49 - 55
Copyright
Copyright © Cambridge University Press 1996

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