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Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans

Published online by Cambridge University Press:  11 January 2019

Xinghao Yao ,
Tailin Wang ,
Huijuan Wang ,
Hongwei Liu ,
Shenghao Liu ,
Qingang Zhao ,
Kaoshan Chen  and
Pengying Zhang
Xinghao Yao
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan 250100, China
Tailin Wang
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Huijuan Wang
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan 250100, China
Hongwei Liu
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan 250100, China
Shenghao Liu
Affiliation:
Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Qingang Zhao
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China
Kaoshan Chen
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan 250100, China
Pengying Zhang*
Affiliation:
National Glycoengineering Research Center and School of Life Science, Shandong University, Jinan 250100, China Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan 250100, China
*
zhangpy80@sdu.edu.cn
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Abstract

Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, six genes (Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 and Pn847) were identified in the Antarctic moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence alignment and three-dimensional structure analysis revealed the conserved amino acid residues of the enzymes of the chalcone synthase family, including three catalytic residues (Cys164, His303 and Asn336) and two substrate recognition residues (Phe215 and Phe265). Phylogenetic analysis indicated that PnCHS088, PnCHS444 and PnCHS768 might be chalcone synthase but that Pn021 is more like stilbenecarboxylate synthase. These genes were located at the transition between fungi and advanced plants in the phylogenetic tree. In addition, real-time PCR analysis revealed that the expression profiles of the six P. nutans genes were influenced by diverse abiotic stresses as well as by abscisic acid and methyl jasmonate. The results presented here contribute to the study of the CHS gene family in polar mosses and further reveal the mechanisms underlying the adaptation of mosses to extreme environments.

Keywords

abiotic stressextreme environmentflavonoids

Information

Type
Biological Sciences
Information
Antarctic Science , Volume 31 , Issue 1 , February 2019 , pp. 23 - 33
Copyright
© Antarctic Science Ltd 2019 

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Footnotes

†

joint lead authorship

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